Celiac disease (CD) is an autoimmune disease, induced by an immune mediated reaction in the small intestine after ingestion of gluten and related prolamines. There is a strong genetic linkage and most important are the genes that encodes for HLA-DQ2 and HLA-DQ8. The European Society for Paediatric Gastroenterology, Hepatology and Nutrition (ESPGHAN) has developed new guidelines for investigation of CD in children and adolescents, which include genotyping for HLA-DQ2 and HLA-DQ8. Therefore, the clinical microbiological laboratory in Västmanland wants to introduce a method for analysis of these genes. Microarray is a newly developed method for determination of the alleles HLA-DQA1 and HLA-DQB1 that encodes for the ?- and ?-subunits of the HLA-molecules.

Rektalcancer drabbar cirka 2000 personer i Sverige per år. Trots förbättringar i preoperativ utredning och behandling, utgör fortfarande lokalrecidiv (lokalt återfall i bäckenet) ett allvarligt problem. I dagsläget överlever inte majoriteten av patienterna som får ett lokalrecidiv. För närvarande finns det inga kliniskt introducerade prognostiska vävnadsmarkörer för risken att utveckla ett lokalrecidiv. Identifieringen av en sådan markör skulle kunna leda till att en individuell behandlingsplan skapas för patienten, både primärt och postoperativt samt för uppföljning.

Celiac disease (CD) is an autoimmune disease, induced by an immune mediated reaction in the small intestine after ingestion of gluten and related prolamines. There is a strong genetic linkage and most important are the genes that encodes for HLA-DQ2 and HLA-DQ8. The European Society for Paediatric Gastroenterology, Hepatology and Nutrition (ESPGHAN) has developed new guidelines for investigation of CD in children and adolescents, which include genotyping for HLA-DQ2 and HLA-DQ8. Therefore, the clinical microbiological laboratory in Västmanland wants to introduce a method for analysis of these genes. Microarray is a newly developed method for determination of the alleles HLA-DQA1 and HLA-DQB1 that encodes for the ?- and ?-subunits of the HLA-molecules.

This Master thesis discusses the development of a virtual human soft tissue object for use in exercise applications of various medical procedures.The soft tissue object to be developed shall look like, feel like and behave as living human tissue. It shall give the user a realistic feeling of weight, softness and roughness, and shall behave in a correct way for exercise purposes. The soft tissue model is to be used in a three dimensional stereo graphic environment with haptic feedback. A prototype for practicing the procedure of inserting a catheter is built. The prototype is used to evaluate the potential of the virtual environment for medical exercise applications, and to assess the realism and usability of the soft tissue object.

Injuries to the suspensory ligament represent a huge problem in equine veterinary medicine. As many of the affected horses are elite-competition athletes, there are high expectations on veterinarians to make these horses fully functional again. The suspensory ligament has several features which makes the healing process complicated. So far no treatment has shown to optimize the healing process. This has led to the testing of many new treatment methods, in order to find a consensus for the injury.

Mammary tumors are the most frequent type of tumors in bitches in Sweden. Many studies have been published concerning mammary tumors, but there is still much to be learned about this disease. One has, since some time ago, had the knowledge that addition to or removal of methyl groups to genes can inhibit or activate gene-expression. Methylation and demethylation is a normal phenomenon in the body and it is essential for the development and survival of the individual. The risk of developing a tumor disease is increased if the global methylationstatus is changed; if oncogenes demethylates (activates) or if tumor suppressor genes methylates (inactivates). In the human medicine one has seen that there are differences in methylationstatus between different tumors. In this study tumor and normal tissue from ten dogs were examined with regard to methylationstatus.

Background: Mastitis in dairy cattle is a common ailment worldwide. A cause of mastitis can be bacteria such as Escherichia coli. Mastitis is not a deadly ailment and sometimes the dairy cows show no symptoms but if certain virulence genes are present in the bacteria that cause the mastitis, the bacteria can be transmitted to humans and cause severe diseases. The potential presence of enterohemorrhagic Escherichia coli (EHEC) in particular would be a major concern for human health.Aim: The aim for this study was to analyze the presence of virulence genes known to be present in E.coli strains isolated from dairy cows with mastitis in Sweden.Method: A Qiagen BIO ROBOT EZ1 was used to purify DNA from 90 bacterial cultures. A panel of virulence genes were amplified and biotinylated from the purified DNA by PCR and an E.coli based DNA microarray was used to detect presumed virulence genes in E.coli.Result: There were no samples that had all the genes traditionally used to classify E.coli as EHEC or potential EHEC.

The determination of the optical properties of biological tissue is an important issue in laser medicine. The optical properties define the tissue´s absorption and scattering behaviour, and can be expressed by quantities such as the albedo, the optical thickness and the anisotropy coefficient. During this project, a measurement system for the determination of the optical properties was built up. The system consists of a double integrating sphere set-up to perform the necessary reflection and transmission measurements, and a computer algorithm to calculate the optical properties from the measured data. This algorithm is called Inverse Adding Doubling method, and is based on a one-dimensional transport model.

Background: A Balanced Scorecard can be used as a tool for communicating and presenting the vision and strategic goals of an organisation in a way that is easy to understand. Implementing a scorecard will support the learning process and dialog concerning the strategy of the organisation. It will also increase the employees understanding of the organisation and motivate them to reach the strategic targets.Purpose: To evaluate and describe employees opinions of a Balanced Scorecard that has been created by the management group from a management point of view. From that, our aim is to recommend our case company, Swedish Tissue, how to implement their Balanced Scorecard. We will also give more general recommendations on how to implement a scorecard.Method: The conclusions in this thesis are the result of a case study.

The aim of this study was to describe non-surgical treatment options for the diseases mucositis and peri-implantitis found in present literature. The literature review also illustrates the effect of the treatment opptions on soft tissue with mucositis and peri-implantitis surrounding dental implants. The study reviews twelve scientific articles that have been processed from the aim of the study. The result shows six therapy options, Er:YAG laser, air abrasive, ultrasonic device, hand instruments, hand instruments in combination with antibiotics and hand instruments in combination with clorhexidin. The following methods are described according to how they are used and how they affect the soft tissue surrounding dental implants.

The aim of this study was to describe non-surgical treatment options for the diseases mucositis and peri-implantitis found in present literature. The literature review also illustrates the effect of the treatment opptions on soft tissue with mucositis and peri-implantitis surrounding dental implants. The study reviews twelve scientific articles that have been processed from the aim of the study. The result shows six therapy options, Er:YAG laser, air abrasive, ultrasonic device, hand instruments, hand instruments in combination with antibiotics and hand instruments in combination with clorhexidin. The following methods are described according to how they are used and how they affect the soft tissue surrounding dental implants.

The following project has been made in collaboration with, Denator AB, a biotechnology enterprise. One-dimensional electrophoreses have been used to analyze protein contents of tissue samples. Tissue samples have been either treated according to the company´s newly developed tissue treatment technique or not treated at all and comparisons between these samples have been made. In order to see differences in protein patterns between samples more clearly, a conversion method has been developed where electrophoresis gel visual patterns are used to produce curves, similar to densitometer curves.Denator AB would like to know if the proteins in their treated samples will change visibly over 48 h when incubated at room temperature. This has been investigated using the above mentioned method.

Basal cell carcinoma (BCC) is the most common non-melanoma skin cancer. It can be divided in different subtypes. The most common type is superficial and is most frequently occurring on the trunk and on the face. In severe cases it can be difficult to differentiate basal cell carcinoma from other skin tumors, therefore a skin biopsy is conclusive diagnostic method. In terms of treatment, curettage is a common non-surgical treatment of basal cell carcinoma.

Lameness from the carpus is not unusual in the horse. Multiple soft tissue structures such as tendons, ligaments, the joint capsule and synovial structures can be the originating source. All these structures can be examined with ultrasound, although a description of a systematic examination method is lacking. That is ? which structures, what they look like in an ultrasound examination, how to look at them in the best way, and in what order.

Feline Odontoclastic Resorptive Lesions (FORL) is a painful disease that affects feline teeth and that is increasing in prevalence. The ethiology is still rather unknown. However, research on the ultra structure of the feline tooth may help us understand why FORL is so prevalent in cats and not in other species. Since the ethiology is still unknown, there is yet no profylaxis. The pathogenesis is rather well known; the hard structure of affected teeth gradually undergo odontoclastic resorption and resorptive lesions are often partly replaced with bone- or cementum-like tissue.